Jamaican sprinter Nesta Carter’s appeal against disqualification from the 2008 Beijing Olympics was denied by the the Court of Arbitration for Sport Thursday, stripping Usain Bolt of one of his nine goal medals.

According to the Associated Press, a fresh analysis of Beijing samples by the International Olympic Committee in 2016 resulted in Carter testing positive for the stimulant methylhexaneamine. The ruling comes nearly ten years after Carter ran the opening leg of the 4×100 relay team Bolt ran third on, helping Jamaica to the win in a world record time of 37.10. Jamaica was disqualified and stripped of the relay title eight years later, and the ruling had been held up in appeal until Thursday.

The CAS panel said it “could not accept any of the arguments raised by Nesta Carter contending that the test results should be ignored or the IOC (disciplinary) decision should otherwise be overturned for certain alleged failures.”

Jamaica’s disqualification means the gold medals will go to the Trinidad and Tobago team of Keston Bledman, Marc Burns, Emmanuel Callender, Richard Thompson and Aaron Armstrong. The IOC should also upgrade Japan to silver and Brazil to bronze. The disqualification tarnishes Bolt’s perfect Olympic record  of three gold medals in the 100, 200 and 4×100 at three consecutive games in 2008, 2012 and 2016.